Basic Tutorial [- Demo data -]

The Basic Tutorial gets you up to speed quickly with key concepts in FlowJo. A common titration experiment is used to demonstrate some of the basic analysis features of FlowJo. In the tutorial, a FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. You can watch one of our application scientists demonstrate the Basic Tutorial (about 15 minutes), and you can follow along with the PDF (29 graphically enhanced pages).

Basic Tutorial Chapters:
1. Flow data and the FlowJo Workspace.
2. Data Analysis in detail (population gating and statistics).
3. Applying the analysis to Groups of samples.
4. Verifying/modifying gates.
5. Generating a graphical layout report.
6. Generating a table of statistics.

If you wish to skip steps, we provide pre-made Workspace files that correspond to each analysis step. The demonstration data and Workspace files can also be found in the Basic Tutorial folder on the FlowJo CD. See also www.flowjo.com/home/tutorial.html.