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Any gate can be made "Magnetic" by clicking on the appropriate option in the Graph Specification Window. Once you make a gate "Magnetic", FlowJo moves it until it accomodates the maximum number of events--i.e., it relocates to where the events are. When you copy a magnetic gate from one sample to another, it is automatically readjusted. If a parent gate is modified, then the magnetic gate is again recalculated to accomodate the maximum number of events. This is useful for instances where the population may move slightly from sample to sample and you don't want to have to individually adjust each sample's gate. Gates which are magnetic are drawn with an arrow in the graph. The arrow is drawn from where the gate was originally set to where the gate has been moved by FlowJo. Gates which move a long distance should be checked for correct positioning! Magnetic gates are, in every other way, exactly like normal gates: you can create subsets and add statistics to populations based on magnetic gates. |
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