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A layout is a created by adding objects to a blank canvas. The objects may be rectangles, lines, text or graphs. Once you have placed objects onto the canvas, you can move them (by clicking and dragging one or more selected items), resize them (by clicking and dragging on the "handles" of a selected item), or align them to each other (by selecting "Alignment..." from the "Layout" menu). The Alignment tool is a powerful tool that even lets you put multiple graphs right next to each other (i.e., abutting axes) to create a compressed view! Rectangles, lines and text can be added via the tool palette, found above the vertical scrollbar on the right edge of the layout window. Select the desired tool, and drag within the editor's view in order to create a new object. [More on Layout Tools] To edit the color, line weight, text styles, or other information about the object, select the object and choose "Get InfoÖ" from the "Layout" menu. (As a shortcut, you can also double click on the object.) This will bring up the Layout Definition dialog box, which provides the user interface for editing any of the graphical characteristics of the object. Which characteristics of the object are editable is dependent on the type of the object. At right is an example of the dialog that comes up when you edit the definition of a text box. Graph Objects To add a graph to a layout, drag the node representing the population or sample from the Workspace window and drop it into the layout. This will create a new graph object in the layout editor. As with other objects, you can use the Get InfoÖ command (or a double click) to edit the characteristics of the graph. The set of characteristic of a graph are substantially different from other layout objects, more closely resembling the floating palette that is used to stylize the graph windows. There are popup menus showing the X and Y axis parameters, as well as others to set the graph type and the optional parameters to governing the look of the resulting plot. In the case of histograms, it is also possible to fix the minimum and maximum values on the Y axis. This will enable you to prevent the axis from being recalculated for each frame in the layout, and make it easier to compare successive frames of a multi-sample layout. More description on these items can be found in the graph window's documentation of the graph specification floating palette. An additional item found in this dialog is the Control popup. This will let you specifiy a graph, or layer in an overlay graph, to be a control. Controls always show a display of their original sample, regardless of what sample is currently being shown in the layout. This provides you a way to create layouts that show multiple samples, all compared to an original or control sample. [Top] The second pane of configuration in the Layout Item Definition dialog is called Annotate, and governs the additional annotation that (optionally) accompanies a graph in a layout. The first setting is whether the annotation is desired at all. If you uncheck this box, then FlowJo will not generate a text box associated with this graph. Assuming that it is checked, you can optionally have the annotation include the graph's title, the name of the sample, then name of all the gates leading to this population (the full name or full path), as well as statistics showing the frequency and/or the event count. There is also an additional text box that will let you include any extra information you may want to display in the layout. Other settings available in this dialog include the abiltiy to suppress either axis. This is useful if you are producing a layout with a large number of graphs and you want to save space by compressing the graphs close together and only label the outside axes. You can request the graph to show its ancestry, meaning that all the parent populations and gates used to define this population will be shown as part of this graph. If this option is chosen, then you can also choose whether to show the ancestry horizontally across the top of the graph, or vertically along the left of the main view. You can also choose whether gates that are defined on this population should be shown. If the graph is an overlay, then you may choose whether the legend will be visible or hidden. |
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