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FlowJo has several analysis platforms that provide
capabilities beyond the gating and statistics used for most simple
data analysis. All analysis platforms are fully integrated
into the FlowJo paradigm: i.e., they can be easily replicated
to multiple samples (usually, by using Groups),
they are easy to edit, and all operations are saved with the Workspace
for your permanent record.
FlowJo has the following analysis platforms:
Kinetics.
The kinetics platform gives the ability to analyze your data as
a function of time during collection. If you did not collect
a "Time" parameter, FlowJo will add one for you. Kinetics
analyses provide you a powerful tool for computing parameter statistics
as a function of time and exporting the data or graphics, or statistics
computed as a function of time. You can even use the Layout
Editor to overlay time-series graphs to compare different samples.
Cell
Cycle Analysis. FlowJo gives you
a sophisticated, yet easy-to-use interface to model DNA/Cell cycle
data. You can simultaneously compute several different standard
models, with the ability to constrain different model parameters
with the click of a mouse.
Calibration.
Using this platform, you can calibrate any of your fluorescence
parameters. The platform can use a calibrated bead set as
a standard, a stained sample as a reference, or numbers that you
enter to define the calibration manually. FlowJo creates a
calibrated parameter and displays all graphs and statistics in calibrated
units such as absolute number of molecules per cell.
Compensation.
Sometimes your data is not properly compensated... sometimes, it
is not possible to compensate your data on the machine because of
the complexity of the analysis. FlowJo's compensation platform
makes compensating data much easier than compensation on the machine;
you are always guaranteed a correctly-compensated output!
Derived
Parameters. Let FlowJo calculate
a ratio of two parameters, add a time parameter (in case you want
to do Kinetics analyses), or convert between log and linear scaling.
Movies.
An analysis platform unique to FlowJo: view your data dynamically.
Use the Moview Platform the generate graphs as a function of time
(kinetic analyses), or to generate a graph of one or two parameters
as a function of a third. This unique visualization lets you
uncover subtle relationships in your data that would be impossible
to see otherwise.
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