Intro to SeqGeq®
Second Wednesday of the month at 10:00 AM PST
SeqGeq® analyses data from RNA-Seq and single-cell RNA sequencing (scRNA-seq), which provides the expression profile of individual cells using next-generation sequencing (NGS) technology to provide up to whole transcriptome analysis. Click here to join.
Intro to FlowJo v10
First Thursday of the month at 10:00AM PST
Learn how to use the FlowJo® workspace, including how to load samples (experimental data), statistics, and gates, create groups and analyses, and generate tabular and graphical layouts. Designed for those new to FlowJo or who would like a refresher overview of the program. Click here to join.
FlowJo Ninja Skills
June 21, 2018 at 10:00AM PST
Take your FlowJo analysis skills to the next level with templates, automation, and an update on the latest tools in FlowJo Version 10. Click here to join.
Concatenation, tSNE, and Clustering
July 19, 2018 at 10:00AM PST
tSNE is a dimensionality reduction tool designed for assisting in the analysis of data sets with large numbers of parameters. tSNE produces two new parameters that effectively summarize all of the information contained in all other parameters. To compare different samples using tSNE, you must first concatenated (merge) the individual samples you wish to compare, then run tSNE on the concatenated file to produce a common dimensionally reduced data space. You can then use gating to pull apart the concatenated file into individual samples and compare how each of those samples is represented in the dimensionally reduced data space. To discover phenotypic changes or shifts between experimental conditions, gate the distinct populations you see in the common tSNE plot, use the original parameters to immunophenotype the populations and then compare the prevalence of those populations of between experimental conditions. Click here to join.
CYTO 2018 Research Talk Spotlight
August 16, 2018 at 10:00 AM PST
What Your Cells Are Not Telling You: A Deep Learning approach to Robustly Classify Monolithic and Novel Cell Types in Acute Lymphoblastic Leukemia. Click here to join.
New Functionality in v10
September 20, 2018 at 10:00AM PST
Join this webinar to see new features in FlowJo v10 explained and demonstrated including spectral compensation, improvements to the table editor, and more. Click here to join.
December 20, 2018 at 10:00AM PST
Accurately quantify G0, G1, S, G2, and M phases of the cell cycle in FlowJo v10. Learn about the cell cycle statistics, how to constrain the models, create phase specific gates, and design tabular and graphical reports. Click here to join.
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