Using flow cytometry to track cell generations is now possible, thanks to the introduction of cell tracking dyes. FlowJo presents a graphical display and a table of data on each generation in the subset. This will provide you with information about how many cell divisions have occurred. In addition the FlowJo Proliferation Platform draws gates in order to separate each generation.

*Division Index is the average number of divisions that a cell (that was present in the starting population) has undergone. For example, if half of the cells in the starting population divided and the average number of divisions was 4, the Division Index would be 2.

*Proliferation Index is the average number of divisions that those cells which divided underwent. For example, if the average number of divisions for all the cells was 4, the Division Index would be 4.

*%Divided is the percentage of the cells of the original sample which divided (assuming that no cells died during the culture). For example, if half of the cells in the starting population have divided, the %Divided = 50%.

Gates can be created that divide each generation into separate subsets and as with all other platforms in FlowJo, the Proliferation Node can be applied to groups of samples by dragging.

You can find more Info on Proliferation in FlowJo's online Reference Manual.