You have chosen to unify the analyses belonging to a group. This means that for the selected node(s), the group's version of the gate will be applied to the selected sample. This will be done only for the sample you have selected.

If you want to do this for all samples in the group, click on the group node in the upper part of the workspace window, and then select the "Unify" menu option.

If you wish to avoid this confirmation dialog window in the future, click on the "always use the same answer" box at the bottom left--FlowJo will remember your answer for the duration of this FlowJo session (regardless of the Workspace). The next time you run FlowJo, this dialog will return.

To learn more about groups and group operations, click here.