FlowJo always runs in a fully functional mode. However, in Demonstration mode, you may only input data files that are Demonstration FCS files provided by Tree Star, Inc. There are a variety of demonstration data files that you can use to explore the various capabilities of FlowJo. Demonstration mode can be used on any PowerPC computer.

In order to use FlowJo to analyze your own data files, you must obtain a user license. A free 60-day user license can be obtained from Tree Star, Inc., allowing you to evaluate FlowJo in an unrestricted fashion. User licenses can take the form of either a serial number (specific to a single computer), a single machine dongle, or a given out by a Network Keyserver.

If your site has purchased user licenses in the form of a Network Keyserver, then FlowJo can look for that server machine (to which a Network Dongle is attached). If it finds it, then it can get a user license and execute.

For the kind of Macintosh you are currently running, you will not be able to use a serial number to run FlowJo. Your only options are to use a single machine dongle, access a network dongle via AppleTalk, or to run in Demonstration Mode. FlowJo has already determined that there is no single-machine dongle attached to this computer. (If you have a single-machine dongle attached, then either it is not properly connected, or you have not rebooted after connecting it).

At the current time, you can either instruct FlowJo to search for a Network Keyserver (this can take a few minutes), or to run in Demonstration mode.