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| Any gate can be made "Magnetic"
by clicking on the appropriate option in the Graph
Tools Window. Once you make a gate "Magnetic", FlowJo
moves it until it accommodates the maximum number of events--i.e.,
it relocates to where the events are. When you copy a magnetic gate
from one sample to another, it is automatically readjusted. If a parent
gate is modified, then the magnetic gate is again recalculated to
accommodate the maximum number of events.
This is useful for instances where the population may move slightly from sample to sample and you don't want to have to individually adjust each sample's gate. Gates which are magnetic are drawn with an arrow in the graph. The arrow is drawn from where the gate was originally set to where the gate has been moved by FlowJo. Gates which move a long distance should be checked for correct positioning! Magnetic gates are, in every other way, exactly
like normal gates: you can create subsets and add statistics to
populations based on magnetic gates. The workspace displays the presence of magnetic gates by changing the icon to a magnet, shown in the Lymphocytes: |
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They are supported in both univariate (histogram) and bivariate
gating tools.