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Groups of samples should be created freely!
You should create groups for every different type of analysis and
sample. For instance, if you have collected samples from three different
tissue types, from several individuals, and using four different stains,
you might consider having one group for each tissue type (since each
tissue may have its own lymphocyte gate), and one group for each staining
combination (so that you can add the appropriate gates for each stain
automatically).
In this case, each sample will belong to two groups.
From one group will come the lymphocyte gate, and from another,
the specific staining gate. You could then create another group
for each individual, so that you can view just that individual's
tubes.
When you collect compensation samples, it is convenient
to create a group just for the compensation tubes. These tubes may
have their own lymphocyte gates which are more or less restrictive
than you would use for the general analyses. Also, it is a convenient
way to segregate the compensation tubes from the other tubes when
you display samples in the workspace.
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