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| This time series is from a calcium-flux
experiment. In this experiment, peripheral T cells were stained with
anti-CD markers to identify subsets and loaded with Indo-1. The ratio
of Indo-1 for a gated subset of CD4 T cells is shown as a function
of time. Approximately 30 seconds into the collection, anti-CD3 was
added to stimulate the cells. The time in seconds since the beginning
of the acquisition is shown in the upper left hand corner.
You will notice that about 230 seconds the first indications of calcium mobilization can be seen by the appearance of a peak of high Indo-1 ratio (Ca, X axis). Within 50 seconds after that a peak of high calcium concentration appears (at about 2 on the Ca scale), which quickly resolves into the main, intermediate peak (about 0.7 on the Ca scale), lasting to the end of the collection. In FlowJo, or when you view the movie using a third-party application, you can view the movie frame-by-frame, or use the mouse to scan back and forth over a small time period: this gives you the ability to view small time-slices with high precision so you won't miss anything. View the next sample movie, or go back to the movies overview. |
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