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| This data is from a 3-color immunophenotyping
experiment using CD4, CD45RA, and CD62L (Leu8) to stain human PBMC.
The collection was gated for lymphocytes using forward and side scatter,
and then for CD4 T cells. A pseudocolor plot of CD45RA vs. CD62L is
shown as a function of the CD4 intensity (below).
The bottom graph shows the slice of CD4 intensity that is used to gate the current frame of the movie. For this movie, equal number of events was selected; thus, each frame can have different ranges of CD4 intensity; this is why the bottom and top slices are particularly wide. (To see how this movie would look with equal channel framing, click here.) You will notice several features about the graph as the CD4 intensity is varied. First, the upper left population (CD45RA+CD62L-) only appears at the very lowest CD4 intensities (this is an activated T cell population that has likely downregulated expression of CD4 itself). As the CD4 intensity increases, you will also note that the amount of CD62L (Leu8) per cell for the CD62L+ cells increases as well. Go back to the overview for movies, or to an example Kinetics Movie. |
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