FlowJo encountered an error while generating the animated frames for the batch layout.  This is likely because FlowJo ran out of memory.  The "Tiled Frames" view should still display the graphics properly.

To increase the amount of memory for FlowJo, quit the application.  In the Finder, select the FlowJo application file, and select "Get Info..." from the File menu.  Enter a larger number into the desired memory allocation for FlowJo and then try to make the batch layout again.