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Many of the operations you will perform
in FlowJo are executed by "dragging and dropping." For instance,
to copy an analysis gate to another sample, simply click on the subpopulation
gate, and while holding the mouse button down, drag until the desired
destination sample (or subpopulation) is highlighted; then let go
of the mouse button. FlowJo applies the gate to that sample, creates
a new node and displays it in the workspace.
Likewise, analysis nodes can be dragged to the table
editor in order to create tables of statistics,
or to the layout editor to create graphical representations. Analyses
nodes can be dragged to different workspaces (that you have also
opened) in order to apply them to samples in that workspace.
When you click on a second node, the first node
is unhighlighted and the second node becomes highlighted. However,
if you command-click a second node, then both nodes are highlighted.
You can shift-click to select a series of nodes (this only works
if the nodes are siblings; otherwise shift-click is equivalent to
command-click). Any drag operation applies to all selected nodes.
When you drag a node, the default action is that
node itself will be moved; none of its descendants or parents go
along. However, if you hold down the "option" key when
you start the drag, then all children of the node become part of
the drag; if you hold down the "control" key, then all
parent nodes of the selected node become part of the drag. Holding
down both keys takes parents and children. The inclusion of children
or parents applies to all selected nodes.
Whenever you start a drag operation, FlowJo creates
a "drag outline" which represents all of the nodes that
you are dragging. This way, you will have a visual feedback of what
is happening.
When you finally drop your selection onto a node,
all of the selected nodes are applied to the destination node: i.e.,
they all become children of the destination node. (Note that if
you selected parents with the node (control key), then the highest-most
parent is the one that becomes the immediate child of the destination
node. Generally, you will only do this operation when you drag onto
the sample-level).
If the nodes you are dragging already exist in the
destination, then you will be asked what to do: you can replace
the existing nodes with the ones you are copying, you can duplicate
the nodes you are dragging (the duplicate nodes will have a "-2"
appended to their name), or you can elect to retain the nodes in
the destination sample. The latter is useful when you want to copy
a whole tree with new analyses onto a sample which already has some
of the gates, but you want to change the sample's versions of the
gates. These options are more fully discussed in the pages on replacing
existing nodes.
All of this is rather complex to describe... an
example of the
different kinds of operations will better
help you understand the ramifications of the various dragging and
dropping operations.
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