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As a simple example, we will look at
compensating a workspace which has five tubes collected: three compensation
controls (one each for FITC, PE, and Cy5PE), and two PBMC samples
that were stained with all three reagents. Our workspace shows these
five samples, and the group that was created to hold this experiment:
For
organization, we create a new group to hold just the compensation
samples, and drag the three compensation samples into the group.
Selecting this group results in only those samples being displayed
as in the window below.
The
next step is to define the gates for
each of the singly-stained subpopulations.
Go back to the overview
of compensation. |