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 The Layout Editor
The Layout Editor is an incredibly powerful tool in the creation of both analysis and publication graphics.  It is designed to create "layouts" which can contain one or more graphical objects, which include rectangles, lines, text and graphs.  It works like a page layout program, but the graphs are live and will recalculate with any change in the data or gating.  The page describing the Layout Editor controls explains the tool palette, commands and options associated with the editor. The page describing layout elements also contains useful information about the myriad of features within FlowJo's presentation capabilities.

Using the Layout Editor, you can generate graphical reports that can do any of the following:

  • Show multiple different graphs from the same gated subset
  • Create a report mixing graphs from different subsets or samples
  • Overlay dot plots, histograms, or Kinetics analyses
  • Mix statistics, text items, boxes, lines, and other items with your graphs
  • Generate batch reports with complex layouts created for each sample in the workspace

Layouts are "live", in that the graphic window is updated whenever any of the gates or statistics change (because you moved a gate, or change an analysis, etc.).  You never have to worry about whether to update the reports or not; FlowJo will do so automatically!

The Layout Editor knows how to iterate, that is, to create the same layout for many different samples.  It can iterate over every sample in a group, or via "Panels" where graphs from different samples are combined in a single layout.

Iterated layouts, or stacks are displayed in a special "stack viewer" window.  You may view stacks, either as Tiled Frames, where the frames are laid out on a two dimensional grid, or as Animated Frames, where time is used as the third analytical dimension, and the frames are played as a movie.  The stack viewer can save, print or export the frames, as well as save them in HTML for export to the web.

Because a layout contains many different graphs and therefore can take a long time to generate, FlowJo supports placeholders to make the editor more responsive while creating the layouts.

The best explanation is to walk through a simple example of using a layout to check the applicability of a gate across a group of samples.  A more involved example is taken from the FlowJo tutorial.

See Also:  Workspace, Table Editor, Groups

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