Other pages talk of iteration, a process of repeating analyses for all members of a group.  This page describes what you can do with the results.

There are several potentially interesting things you might want to do with this data:

• Print it one layout per page as a report 
• Print it at a minimum readable size for summary analysis
• View it as tiled graphs onscreen
• View it as a movie onscreen
• Save a standard Pict file for further processing / publishing
• Create a website showing all images
• Create a website including the movie

There are several output types for batched reports: a new layout, a movie, a web page, and a preview (tiled) report. In addition, you can directly print a report. The Stack Viewer is used to display the Preview or Tiled report.  As the layout editor iterates, it builds a series of frames.  The collection of all the frames is called a stack.  The preview report displays the stack of frames in two different ways:

• Preview (Tiled) Frames:  a grid of images that can be printed.
• Animated Frames:  a movie player that can quickly skim a stack via QuickTime(r) movies

Both views support outputting the graphic display to disk, clipboard, or printer.  These functions can be accessed by the menu commands (under the "File" menu), the command-key equivalents (command-S, command-C, and command-P, respectively), or by clicking on the appropriate icons near the top right of the Stack Viewer window.

Preview Report

Below is a screenshot of a Stack Viewer in Tiled Frames mode:

Tiling Options

The tiling pattern on the screen is controlled by the popup menu in the top bar.  You can choose to lay out your frames by the number of columns you desire.  One up, Two up, Three up and Four Up are offered, and should cover most cases.  The final item in the menu will bring up a dialog containing more options.

The Custom Layout Options dialog handles special case scenarios where you want to determine the layout from a fixed number of rows instead of a fixed number of columns. It allows you to order the graphs from top to bottom instead of from left to right.

You can enter a value for either dimension, and the other side will be automatically recalculated to accommodate the number of frames in your stack.

"Order successive samples" is a way to determine how the frames are sequenced, as illustrated by this picture (left).

Order Across:  Each frame is placed to the right of the one before it, until the edge (as determined by the number of columns in the dialog) is reached, and then a new row is started.

Order Down:  Each frame is placed below the one preceding it, until the bottom (as determined by the number of rows in the dialog) is reached, at which point a new column is started.

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Printing

One of the most annoying aspects of large analyses is having to manually piece pages back together because the layout program can't conform to the printer's page breaks.  FlowJo avoids these problems by printing a specific number of tiles per page. When you click the printer button from a Tiled report, the Choose Print Layout Options dialog will open and allow you to choose the number of tiles per page and many other printing options.

Animated Frames

In addition to having the ability to tile frames for efficient printing, FlowJo also has a unique tool for visualizing this large accumulation of information.

The human eye and brain are better suited for finding patterns changing through time than they are in juxtaposing patterns in a two dimensional grid, or in constructing them in a three dimensional space.  If you are searching through a large number of samples looking for patterns, similarities or trends, this animated view may give you a new approach to try.

Since the data for the animated frames and tiled frames are based on the same graphs, you can quickly switch between the views without have to recompute extensive numbers of graphs. As you switch between the view, you'll see that many of the controls are different.

Across the bottom of this window are the standard controls used to play a quicktime movie.  The leftmost arrow is the play button.  The right side controls move to the previous or next frame in the movie.

There are also keyboard shortcuts for each of these actions:

The space bar (or double clicking the frame) will play the movie.  The left and right arrow keys move to previous and next frame.

It is possible to export movies to other Mac programs or web sites by dragging the image from the window to the Finder.  Shift click on the thumb of the scroller to select a subrange for export.