Print Preview

Once you have created a layout in the editor, you can direct FlowJo to produce a batch analysis.  That is, to create the same picture, using each of the samples or values of the iteration attribute in the current group.

Iteration is a process of repeating analyses for all members of a group. Other pages describe it in detail, the current page describes what you can do with the results.

There are several potentially interesting things you might want to do with the results of a batch/iteration process:

• Print it, one layout per page, as a report. 
• Print it at a minimum readable size for summary analysis.
• View it as tiled graphs onscreen.
• View it as a movie onscreen.
• Save a standard PDF file to disk for further processing / publishing.
• Create a Webpage showing all images.

Printing

To preview your print job, select File > Print Preview.

One of the most annoying aspects of large analyses is having to manually piece pages back together because the layout program can't conform to the printer's page breaks. FlowJo avoids these problems by printing a specific number of tiles per page. When you click the Batch button in the Layout Editor, the Create Batch Report dialog will open and allow you to choose the number of tiles per page and other printing options.

Animated Frames

In addition to having the ability to tile frames for efficient printing FlowJo also has a unique tool for visualizing this large accumulation of information. Click the Make Movie button and each page of your batch will become a frame in a QuickTime movie.

The human eye and brain are better suited for finding patterns changing through time than they are in juxtaposing patterns in a two dimensional grid, or in constructing them in a three dimensional space. If you are searching through a large number of samples looking for patterns, similarities or trends, this animated view may give you new insight.

Since the data for the animated frames and tiled frames are based on the same graphs, you can quickly switch between the views without have to recompute extensive numbers of graphs.

Across the bottom of this window are the standard controls used to play a quicktime movie.  The large arrow is the play button.  Other arrows move to the previous frame, next frame, first or last frame in the movie.

There are also keyboard shortcuts for each of these actions: The space bar (or double clicking within the frame) will play the movie.  The left and right arrow keys move to previous and next frame. Click for more information on the scope of the Movie tools.