Preferences
There are a number of default behaviors of FlowJo which you can alter. These are modified through the Preferences dialogs, shown below. To get to these dialogs, select Preferences under the Edit menu in the Workspace, Graph window or Layout Editor. The Workspace tab is explained just below. The Administrative tab is explained below that.
Templates and workspaces are now delivered with the preferences preset. A workspace may contain a preference element, or any subset of the elements and attributes supported in FlowJo. The setting and saving of preferences is primarily accomplished locally in each of FlowJo's tool windows. If you change a setting and want to keep the change for future use, go to Edit > Save State to Preferences. New operations of the same type will use the settings you saved. On exit, workspace, templates and layouts carry their current font information with them.
Workspace Tab

There are two tabs with preference settings. Above is the Workspace tab. Keywords lets you choose how FlowJo will name .FCS files as they are loaded. The Default setting uses the .FCS Sample ID keyword. Use data file name will use the name that appears in the computers file manager. If you choose Keyword Combination you can select one or more keywords from the drop-down list. If you choose more than one they will appear together in the sample list. For example Sample ID plus $DATE will produce a name like Sample 3 29-Jul-05.
Next you can have FlowJo warn you when the percentage of events compressed on the axis of a graph, as displayed with the current settings, exceeds a percentage you set.
Remember Open Windows at Startup will instruct FlowJo to reopen the set of windows that were in use when it last exited the program.
Check the Remind to save every x minutes if you want FlowJo to open a dialog reminding you to save your work.
Save with incrementing version number automatically adds saves your workspace without overwriting earlier work. Instead a version number is added to the name and both versions remain accessable on your hard drive.
Child Populations
Display other parameters than in gating plot Checking this box causes FlowJo to display gated subpopulations with the axes labeled differently than the parent population. If there are other parameters to choose from, FlowJo will choose new parameters.
Show child graph:
Same as Parent Graph When you open a gated subpopulation, the graph type is the same as the parent's graph type.
Default set in preferences Select this option to choose the type of graph a subpopulation should use. Open a subpopulation graph, change it to the desired type, then select Edit > Save State to Preferences
Blank graph Child population graph will be blank.
If you wish to return preferences to their original state, select the Reset to Defaults button.
Administrative Tab

Email Options Our email address is entered by default. Enter your email address in the second box. Check the Email problems to FlowJo so that, in the unlikely event that the program should become unstable, an email message to us will be generated automatically with information that will help us prevent a repeat of the problem.
Hardware Address Displays the hardware address of the machine upon which FlowJo is running. This number is useful to us when we create a new license for a user. A FlowJo license is generated for a particular computer using this number.
WiFi HW Address This box will display the hardware address of a WiFi wireless ethernet adapter if one is connected to your machine. It is shown here to prevent confusion between the computer's address and the adapter's address when issuing a license.
Serial Number This is the box where you will cut and paste a serial number mailed to you from us. Once entered, FlowJo will run as licensed software.
Proxy Settings Button... If you use a proxy to access the internet, this button will open up a dialog where you can enter your Host and Port settings. This will allow FlowJo to access the World Wide Web for help files and other references.
The Mute sounds button lets you shut off FlowJo's sound signals.
Select Don't Check For New Versions of FlowJo to prevent pop-up notification when a new version appears on our Web site.
Select Check For New Beta Versions of FlowJo to be notified when a new beta version of FlowJo appears on our Web site.

