The statistics displayed in a column on the graph will depend on which model and options are computed, but both will include the fraction of cells in G1, S, and G2, the means and CVs of the G1 and G2 peaks, and the fraction of cells below G1 and above G2.
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By checking the Split S Phase Stats checkbox from the Models disclosure area, a percentage for cells in S1, S2, S3 and S4 will be provided.
You can add other statistics by clicking on the statistics button. You can remove a statistic by clicking on the Remove Statistic button. Statistics can also be copied, added or removed from Edit menu the of the cell cycle graph window.
In addition, the RMS (root mean square) error of the fit is displayed at the top of the list. If you change the fitting criteria, you may wish to minimize this value as a way to optimize the fit. If FlowJo fails to fit the model(s) to the data, then it will display "Invalid" in the RMS field. In this case, you will want to help FlowJo fit the data by constraining different parameters.
Constraints,
Preferences
Models
Gates
Cell Cycle Workspace with Demo Data
