Picket fencing (broken lines displayed on digital data around zero) can be visually smoothed using FlowJo's display transformation feature. Below is a figure showing a normal plot on the left and a transformed plot on the right. The data is exactly the same - only the scales have been altered to display the double negative population as a cluster. The data below was collected with the DiVa software. The sample was blank beads plus a few contaminants.
When exporting from DiVa, you have two options: "Export FCS" or "Export Experiment". FlowJo will read both formats.
To display Transformation for compensated parameters, select a compensated parameter in the graph's axis label drop down list. Click the transform button on the graph window. 
For more details, including changing Transformation values, click here.
