Gating is the most important aspect of analysis of complex data (like flow cytometric data). Gating refers to the process of selecting a subset of the collected events for further analysis. Subsets can continue to be gated to generate further subsets, until a collection is had of only the cells for which a graphic display or analysed statistic is wanted.
The process of gating simply creates a new population. Naming subpopulations is important. Siblings with the same names are not allowed, lest confusion arise. In general, avoid using duplicate names of subpopulations within any given sample.
To learn more about gating, view the tutorial video on the right.
