Add a Value Series Keyword

Think of a value series as a generic way to build a pattern that corresponds to your patient ID, staining protocol or any other information that differentiates your tubes, instead of (or in addition to) using keywords. FlowJo can use keywords to organize and batch through your data but in the case that the keywords are missing or do not accurately reflect the type of operation you are looking to perform a value series can be used.

An example of this would be collecting your data without providing Patient ID information. Let's say you have 4 patients, with 4 tubes per patient, each tube containing a different staining protocol. A value series could be constructed to repeat the basic pattern: 1234, 1234, 1234, 1234. Then, by sorting according to value, the workspace could be organized to put all the 1s together, 2s together, etc. which allows you to group by Patient ID and create tiles for batching that reflect that pattern as well.

In addition, FlowJo allows you to assign keyword type values to the series you create to make the series have more biological significance.

If the FCS files themselves do not contain keywords that help organize them, add a new keyword within FlowJo, assign values to the keyword for each sample, and use your new keyword to sort or batch tubes accordingly.

First, add a new keyword column to the Workspace. Go to Workspace > Add Keyword...
Choose a name for the new keyword. This adds a new column with that name to the Workspace.
Click in the first cell of the new column to activate it.
From the main menu select Groups > Create Keyword Value Series... The following dialog appears. Mouse over the red dots for more information.

To apply a text label as a series, create an equation to match the pattern of your samples. Then type the desired text labels into the text boxes in the Keyword column on the right. More on text labels in value series.

The value series need not be natural numbers i.e. 1,2,3,4... Using this tool you can create a series according to the equation shown. Choose a Starting Value, choose the amount by which each number of the series will increase (Increment) and add a Multiplier for each successive number if desired. The resulting series is displayed at the bottom of the window under Generated Series. The Rules section tells FlowJo how to enter the value series into the Keyword Column. The Fill number tells FlowJo how many numbers from the series to use. The Repeat Value number lets you repeat each number in the series as often as you wish. The Start Over value tells FlowJo to restart the value series after counting the number of tubes you enter. Here you can preview the series you have created using the tools above. You can associate each number in a series with a word. The word will populate the Keyword column in place of the corresponding numeric value. More...

Flow Cytometry Analysis Software


	Add a Value Series Keyword
V7.5 Manual Table of Contents Workspace Graphs Platforms Output Techniques Menus Preferences Related Links Add a Keyword Series Groups Statistics Node Names Batching Iteration	Think of a value series as a generic way to build a pattern that corresponds to your patient ID, staining protocol or any other information that differentiates your tubes, instead of (or in addition to) using keywords. FlowJo can use keywords to organize and batch through your data but in the case that the keywords are missing or do not accurately reflect the type of operation you are looking to perform a value series can be used. An example of this would be collecting your data without providing Patient ID information. Let's say you have 4 patients, with 4 tubes per patient, each tube containing a different staining protocol. A value series could be constructed to repeat the basic pattern: 1234, 1234, 1234, 1234. Then, by sorting according to value, the workspace could be organized to put all the 1s together, 2s together, etc. which allows you to group by Patient ID and create tiles for batching that reflect that pattern as well. In addition, FlowJo allows you to assign keyword type values to the series you create to make the series have more biological significance. If the FCS files themselves do not contain keywords that help organize them, add a new keyword within FlowJo, assign values to the keyword for each sample, and use your new keyword to sort or batch tubes accordingly. First, add a new keyword column to the Workspace. Go to Workspace > Add Keyword... Choose a name for the new keyword. This adds a new column with that name to the Workspace. Click in the first cell of the new column to activate it. From the main menu select Groups > Create Keyword Value Series... The following dialog appears. Mouse over the red dots for more information. To apply a text label as a series, create an equation to match the pattern of your samples. Then type the desired text labels into the text boxes in the Keyword column on the right. More on text labels in value series. The value series need not be natural numbers i.e. 1,2,3,4... Using this tool you can create a series according to the equation shown. Choose a Starting Value, choose the amount by which each number of the series will increase (Increment) and add a Multiplier for each successive number if desired. The resulting series is displayed at the bottom of the window under Generated Series. The Rules section tells FlowJo how to enter the value series into the Keyword Column. The Fill number tells FlowJo how many numbers from the series to use. The Repeat Value number lets you repeat each number in the series as often as you wish. The Start Over value tells FlowJo to restart the value series after counting the number of tubes you enter. Here you can preview the series you have created using the tools above. You can associate each number in a series with a word. The word will populate the Keyword column in place of the corresponding numeric value. More...

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Flow Cytometry Analysis Software

Add a Value Series Keyword

V7.5 Manual

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