When you want FlowJo to repeat a layout you have made, substituting other samples for the current ones, there are several options available. See Batching (overview) Iteration (details)

FlowJo can organize a batch by sample, by stain panel, or by keyword. (Complex batches can be organized by a sequence of these parameters.) Set these preferences with the drop-down menus at the top of the Layout Editor.

If batching is set to a particular Sample name, and a different sample is dragged in to the Layout Editor, FlowJo needs to know how you want to handle this "Iteration Mismatch."

Go to Edit > Preferences and click the Layouts button.

Under Drag Mismatch Policy you have the following options:

If batching is set to a sample name, and a different sample is dragged in to the Layout Editor, this setting tells FlowJo to:

1. Turn Iteration off
2. Change the dragged sample to the one currently specified in the Layout
3. Change the Layout setting to match the new sample
4. Ask the user to choose