Sample Properties

You can access Sample Properties/Information by selecting a sample in the Workspace and choosing Sample Properties from the Workspace menu, or right-clicking on a sample and choosing Sample Properties (dialog shown below).

Sample info displays the FCS keyword header values for the sample you selected in the Workspace, parameter and stain information and any compensation matrix associated with the file. Flow Cytometry files follow an international standard format called FCS. FCS files are composed of two sets of information - fluorescence and scatter values for each event and keyword values describing the sample. FCS keywords hold descriptions of the sample such as the Date, Time, Cytometer, Antibody, Parameter etc. These keywords are written in code, starting with the dollar sign (eg, Date is $DATE).

Visit the ISAC web page for more information on the FCS file format. Click here to view a short description of commonly used FCS keywords. For information about how FlowJo handles FCS files, go here.

FlowJo will also display keywords and parameters that have been added to the Workspace from within FlowJo. To learn how to do this, click here. While these keywords and parameters do not become part of the FCS file itself, they will stay available within the workspace. They can be written into a FCS file that FlowJo will create for you by using the Export/Concatenate function.