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Probably the best way
to understand the Layout Editor is to walk through a simple task to
see the steps involved in producing a series of graphs to check your
data. In this example, we will create a simple analysis to check
the applicability of a light scatter gate across all of the samples
in an experiment.
- Create a new workspace, and populate it with
a group of samples. (Here we show a workspace containing
the FlowJo Tutorial's Experiment 1 data.)
- Create a Lymphocyte gate drawn on Forward Scatter
vs. Side Scatter.
- In the Workspace window, drag the Lymphocyte
population onto the group containing all the samples in Experiment
1.
- Bring up the Layout Editor, by clicking the button
in the
 workspace,
by selecting Layout Editor… from the Windows menu.
- Name the current layout: Lymph Gate Checker
- Drag a top level sample from the workspace into
the canvas of the layout editor.
- Select Get Info… from the Object menu or
use the shortcut of double clicking the graph. From there
you can edit many of the characteristics of the graph.
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1. To generate this graph for all of the samples
in the experiment, click on the Batch button. A dialog will
pop to specify how the layout will be created. Click OK and FlowJo
will generate a new Layout (called Layout-Batch) with a summary
of all the graph from this group of samples.
2. The new layout view shows all of the graphs
laid out on a single large page. The display will be scaled
so that all of the graphs are visible. Scan all the graphs
to see if the scatter gate correctly surrounds all of the Lymphocyte
populations.
The layout is live - meaning that any gate changes
are always shown immediately in the layout. You can save, copy
to the clipboard, and print this report. Drag the intersection
of the page breaks to change the page size and fit all the graphs
on a page.
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