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Most operations in FlowJo are point-and-click oriented rather than menu-based. Thus, there are relatively few menu items specific to FlowJo. Click on each menu item to go directly to that information (all information is contained on this page).

WORKSPACE: [file] [edit] [workspace] [windows] [help] [debug]
LAYOUT EDITOR: [file] [edit] [layout] [align] [object] [view]

 

Workspace > File
New Workspace - create a new workspace
Open Workspace - Open workspace into current window
Open Workspace in New Window - open workspace into new window

Save - Save current workspace
Save As - save current workspace with a different name/different location
Save As Template - turn current workspace into a template
Revert - if you opened a document, made some changes, revert will reset those changes.

Page Setup/Print Preview/Print...

Close - Closes current workspace. If last worskpace is closed, program exits.
Exit - Exits FlowJo
Workspace > Edit
Undo - undo last operation
Redo - redo last operation (after undo)

Cut - remove from LE and place in memory
Copy - copy an item(s) from LE to memory
Paste - place an item from memory to the LE
Clear - remove an item from LE completely
Select all - select all items in the LE
Select None - if you have trouble un-selecting samples, use this option
Select Every.. - useful for selecting every nth sample
Select Eq. Nodes - selects all nodes on this level with this name (ex. all lymphocyte gates)
Collapse All - collapses all disclosure triangles in current workspace

Preferences - launch FlowJo Preferences

Workspace > Workspace

Add Samples - click that to start browsing for your FCS data.
Add Remote Sample.. - allows adding samples over FTP (specify path and file name)
Remove Samples - select a sample and this menu option to remove FCS data.
Add Keyword - allows users to enter their own keywords into the workspace
New Group - creates a new group, brings up the group definition dialog
Edit Group - click on an existing group, then this menu item, brings up group definition dialog for current group
Remove Group - click on a group and this menu item to remove the group
Remove Group Analysis -if you defined a group analysis node, this menu item will remove it (select the node, then select this menu item)

Add Statistics - toggle statistical nodes
Hide/show Statistics - toggle display of statistical nodes in the workspace
Unify Analysis - see the FAQ

Open Graph Window - displays current FCS file/population in Graph Window
Rename... - rename current node (may not work if group owned)
Sample Properties... - display keywords and system info of currently selected FCS file

Edit Columns... - will allow you to define different columns in your workspace
Sort Sample Lists... - just click the column header instead to sort by the values in that column

Workspace > Windows

Open Layout Editor - opens the layout editor
Open Table Ediotr - opens the table editor

Workspace > Platforms

Define Comp Mtx - brings up the compensation matrix definition dialog
Assign Comp Mtx - assigns a compensation matrix to a selected FCS file
Define Transform - opens the transformation definition dialog. This requires a comp matrix.

Derived Parameters - brings up the Derived Parameters dialog.

Workspace > Help

About FlowJo - shows latest version related info
FlowJo website - opens flowjo.com

Serial Number.. - opens serial number help page
Dongle.. - opens dongle help page

FlowJo Tutorials - opens tutorial index page
FlowJo Demo Data - opens demo data index page

Help with Workspaces - launches the website help page
Adding Samples - launches the website help page
About Groups - launches the website help page
Using Statistics - launches the website help page

Create Archive - compresses current workspace and FCS files to a ZIP archive
Email FlowJo- brings up a dialog that allows users to send info and data to Tree Star

Workspace > Debug

How to enable debug menu: http://www.flowjo.com/win/debug.html
log - shows what FlowJo's working on
XML for... returns the XML syntax for various objects in the program
engine interaction - important window to trace reproducible errors
turn on/off engine logging - toggles the logging of the engine.

Layout > File
Save - saves your current workspace/layout.
Page Setup/Print Preview/ Print - for printing. See the print page for more details.
Scale to page - adjust the page size to include everything in your current layout.
Scale to width - adjust the page size to the width of your layout
Scale to height - adjust the page size to the height of your layout.
Layout > edit
Undo - take back last action

Cut - remove from LE and place in memory
Copy - copy an item(s) from LE to memory
Paste - place an item from memory to the LE
clear - remove an item from LE completely
Select all - select all items in the LE

Send to Back - send selected item(s) to the back
Bring to Front - bring selected item(s) to the front
Send Backward - send selected item(s) one level back
Bring Froward - bring selected items(s) one level forward

 Nudge - fine adjustments of the selected item(s)
Layout > Layout
Add Layout - inserts a new layout to the current workspace
Duplicate Layout - inserts a copy of the current layout into the current workspace
Iteration Options - define how your layout should be iterated
Save Layout Settings -
Make Batch - batch files using the current layout/sample group/iteration options
 

Layout > Align
Tops - Aligns the tops of selected objects
Bottoms - Aligns the bottoms of selected objects
Vertical Centers - aligns the vertical centers of the selected objects
Vertical Spacing - equalizes the vertical distance between selected objects
Vertical Spread - equalizes the spacing between the centers of the selected objects
Lefts - alings left edges of selected objects
Rights - aligns right edges of selected objects
Horizontal Centers - alings the horizontal centers of the selected objects
Horizontal Spacing - aligns the horizonal spacing between selected objects
Horizontal Spread - aligns the spacing between the centers of the selected objects
Align to Grid - aligns selected objests to the grid

Layout > Object

Properties - brings up the properties dialog for selected object(s)
Select Original Population - highlights the corresponding file in the workspace
Open Original Graph - opens the selected graph in a graph window
Insert Picture - allows you to insert a graphic file into your layout
Group - groups selected objects
Ungroup - ungroups selected object(s)

Layout > View
Zoom In - makes things appear bigger
Zoom Out - makes things
Fit In Window - adjusts the zoom setting to fit the whole thing in
Show at 100% - shows the layout at actual size
Use Place Holders - speeds things up by blanking out all the objects with 'placeholders'
Toggle Grid - shows/hides the grid
Toggle Page Breaks - shows/hides the page breaks

Layout > Help all the help menu items link to corresponding manual pages on the web.

 
 
 

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