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| The primary statistics that FlowJo computes
are frequencies (percentages). These are calculated for each subpopulation
out of either an ancestor population such as the parent or grandparent
or out of the entire sample. Except for the frequency of parent, which
are always displayed for each subpopulation once it has been gated,
statistics must be added as separate nodes in the workspace window.
Each statistic node holds a single value (a single statistic); it
is recomputed whenever a gate that affects that subset changes. The
full list of statistics includes such computations as Fluorescent
Intensity (either median, mean, geometric mean), Cell Number (count),
Frequency of Total, and Frequency out of any ancestor and many more!.
Statistics can be added to populations in two ways: (1) by selecting one or more populations and clicking on the "Statistic" function button in the workspace window; this adds statistics to the selected populations, or (2) by viewing a population in a graph window and clicking on the "Statistic" button in that window. Both ways brings up the Statistics Dialog window. Statistics are displayed in the workspace window whenever they are calculated. If a statistic node in the workspace does not show its value, then you may request it to be computed. Select any node which has that statistic as a descendant (i.e., the population to which it is attached, or any parent of that population), and select "recompute" from the FlowJo menus. All statistics can be hidden from view by selecting the Workspace menu item (show them by selecting the same menu item again). Statistics can be conveniently organized and exported by the table editor. Statistics nodes can be dragged to the table definition; when the table is computed, then all statistic nodes will be updated and computed. In addition, you can drag statistics into the layout editor. This will create a text box containing the statistic description and the value; it will be updated whenever the statistic is modified (i.e., it is "live"). |
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