From this window you will manage all derived parameters for a sample. You can add any of the five types of parameters (time, ratio, log/linear conversions, or absolute calibration)... you can have as many derived parameters as you wish (but only one time parameter can be defined). In all cases except when you add a linear ratio parameter with a defined scale, then FlowJo computes the optimal scaling factors to display the most events onscale. In some cases, this means that the same derived parameter for different samples may have different scaling. Thus, you should be careful if you copy gates based on such derived parameters between samples to ensure that the gates are appropriately placed.

Each newly-defined derived parameter is given a parameter name corresponding to the type of function with the parameters of the function (e.g., "FL4/FL5" for ratio). Because parameter names for any given sample must be unique, adding a second derived parameter of exactly same type (& parameters) will result in the parameter name being appended with a number (e.g., "FL4/FL5 -2").

The bottom of the window shows currently defined derived parameters; you can choose to delete any or all of those parameters. Of course, if you have any analyses in the workspace that are based on those derived parameters, then they too will be deleted when you delete the parameters.

Go back to the overview on derived parameters.