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When you select
"Edit Columns..." from the "Workspace" menu, then the
dialog below is shown. From this dialog, you can change what information FlowJo shows you about the samples in your workspace.
From this dialog, you can choose from a variety
of different keywords whose values you wish to display in the
workspace. Each FCS data file has two essential components, the
description of the conditions at the time of collection, and the
sets of numbers measured for each event. It is up to the operator
to correctly annotate the conditions using Keywords to organize
this information in each FCS file. Each keyword is designated
with a $abc (non-standard keywords do not have a $). For more
information about the possible keywords that you might see in
the left selection list, click here.
The order in which the Keywords are shown
in the workspace is the same as the order in the list on the right (drag
the keywords to change the order). The "Name" column must always
be displayed. If you select a keyword for which a sample has no value, then
the column is left blank for that sample. For information on annotating
your data-post acquisition, click here.
If you choose to display
"File name" to display, then FlowJo
will add a column which shows the actual file name (on the disk). This can
be different from the keyword value "$FIL", which has the file
name that the data was originally saved as at the time of collection. If
you choose to put the file name in the "Name" column using the Preferences window (workspace preferences),
then the Name column and the File name column will be identical.
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