AutoSpill compensation: AutoSpill is a new algorithm for calculating spillover and producing a compensation matrix developed and incorporated into FlowJo v10.7 in collaboration with researchers from the Vlaams Instituut voor Biotechnologie in Belgium and the the Babraham Institute in Cambridge, UK.
Our exclusive AutoSpread algorithm provides a Spillover Spreading Matrix (SSM) by calculating spillover spreading coefficients with linear models, removing the need for well-defined positive and negative populations in single-color controls.
"Together, AutoSpill and AutoSpread remove limiting constraints of traditional compensation methods, easing the preparation of compensation controls in high-parameter flow cytometry, making errors less likely, and facilitating the practical implementation of ultra high-parameter flow cytometry." —Roca, et al
Proliferation and Cell Cycle models have been updated to better fit data with missing peaks.
The t-SNE derived parameters now remain connected to workspace files after saving. Note: In case derivative files are ever dissociated from a workspace, drag and drop the CSV back onto the data file or right click and choose “Import Parameters from CSV/CLR”.
Figures added to the Layout Editor will no longer lose parameter annotations without user deselecting those features manually or setting them hidden in the preferences.
Total Spread Matrix (TSM) is now available for matrices created in FlowJo. TSM is the Spillover Spreading Matrix (SSM) without normalization to the florescent intensity of the probe.
For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes.
FlowJo Portal license: Updating to the latest version of FlowJo for FlowJo Portal license holders is easy! Simply download the latest version of FlowJo v10 above and sign in with your FlowJo Portal ID.
Dongle: Download and install the latest version of FlowJo v10. Your dongle will automatically authenticate all versions of v10.
Serial Number: Download and install the latest version of FlowJo v10. Your serial number will transfer and automatically authenticate all versions of v10.
Upgrading from v9
Dongle: Download and install the latest version of FlowJo v10. Your dongle will automatically authenticate all versions of v9 and v10.
Serial Number: Download and install the latest version of FlowJo v10. Your serial number will transfer and automatically authenticate all versions of v9 and v10.
If you are unsure of which version your dongle allows access to, call 1-541-201-0022 or email email@example.com for license support.
If your dongle is in good working condition, we can upgrade it electronically. We also offer physical upgrade and replacement which includes a shipping fee.
If you wish to turn in your dongle towards FlowJo Portal credit, we will use the original purchase date of your dongle.
Upgrades do not qualify for dongle trade-in credit towards a FlowJo Portal license.
If you would like more information about your dongle, please call 1-541-201-0022 or email license support with the dongle serial ID of your USB dongle. Instructions for locating your dongle serial ID can be found here under Locating your Dongle ID.
Click here to learn more about running FlowJo on Catalina, the latest Apple OS.