Versions

v10 Requirements

Minimum Requirements

Recommended Requirements

• 2GB RAM

• Core II Duo processor or better

• OSX 10.6.8

• Apple Java Runtime Environment 6. Available at the following link, not viewable through Safari browsers: 

Java for OS X 2015-001

• USB port (dongle option)

• Network adapter for an off-line serial number

• Network adapter and an Internet connection for Site License serial number

•16GB RAM

•Intel i7 quad core processor

Minimum Requirements

Recommended Requirements

•2GB RAM

•x86 or x64 dual core processor

•Windows Vista

•Oracle Java Runtime Environment 6 (bundled with download)

•USB port (dongle option)

•Network adapter for an off-line serial number

•Network adapter and an Internet connection for Site License serial number.

To see which type to download:

Right-click My Computer > Properties

•16GB RAM

•Intel i7 quad core processor

•Windows 10

v9 Requirements

Minimum Requirements

Recommended Requirements

OSX 10.12.2 

Compatible with Mavericks

Native support for G3,4,5 and all Intel based systems.

2GB RAM

OSX 10.12.2+

*OSX 10.12.1 not currently supported*

8GB RAM or higher

Windows

10.4.1

Released 11-30-17

New Features

  • Search bar added to parameter selectors in the graph window.
  • Color mapped (aka “heat map statistic”) parameter labeling in layouts.

Bugs Fixed

  • Proliferation Platform Improvements
  • Stopped Compensation Wizard matrix re-calculations (ad infinitum).

More Info

For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes.

10.4

Released 09-15-17

New Features:

  • FlowJo Envoy integration now allows for seamless access to data in FlowJo Envoy workflows.
  • Hotly anticipated color mapping within the graph window, to illustrate a third parameter there is now available.
  • Signal spreading matrix (SSM) now available in the Compensation Editor.

Bugs Fixed:

  • Keywords now import into text-boxes in the Layout Editor.

Warnings:

  • When saving an ACS file from an FlowJo Envoy based workspace, the default location prompt is a temporary cache folder, which will be deleted when FlowJo is closed!
    • Workaround: Save FlowJo Envoy based workspace ACS files to a known, stable local directory.

More Info

For more about features and a list of known issues, see the exhaustive release notes.

10.3

Released 05-09-17

New Features:

  • We are happy to announce that the Proliferation Platform is here!
  • We have revised the Logicle Transform implementation. Existing workspaces with gates using this transform may need to be adjusted.

Warnings:

Because FlowJo® now uses Java 8, we no longer support Mac OS 10.6.8 (because it does not support Java 8).

Major Changes:

  • Proliferation Platform: FlowJo now supports Proliferation.
  • Revised Logicle Transform implementation to synchronize the user interface and the correct display ranges.
  • Java 8: FlowJo now uses Java 8, which brings a number of security and reliability improvements. This capability is now is distributed with the FlowJo install on both Windows and Mac.
  • Mac OS Sierra (10.12): This OS is now fully supported.

10.2

Released 09-02-16

Note: This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo.


FlowJo can now use plugins that can perform a variety of functions, including the creation of populations, creation of derived parameters, and actions when opening or saving a workspace. 


The open plugin architecture of 10.2 allows access to cutting edge tools that can be integrated into your current analysis workflows, and we will be releasing developer documentation so any tool can be integrated into FlowJo. FlowJo 10.2 includes a set of tools for discovery including downsampling, tSNE and CellOntology. You'll find these as 'plugins' in the populations band -- each offers one-click access to powerful tools to reduce any number of parameters to two and to query a database to name unknown populations.

New Features:

•Increased the number of parameters that FlowJo can analyze to 1000

•Added a field to allow specification of R's path in prefs (Diagnostic Panel)

•Better interface for Licensing Preferences Panel in the case where a computer has many hardware addresses.

•Updated EULA to raise the awareness of the fact that you may not install FlowJo on a VM.

•Unchecked 'Display other X/Y parameters than in parent plot' as default in Gates Preferences

•Removed the option to downsample when concatenating files. (Downsample before you concatenate if desired.)




Full Release Notes

7.6.5

Released 09-12-11

Minimum Requirements

Recommended Requirements

Windows XP - Windows 7 

512MB RAM

1280x1024 (19 inch) screen

network connection (for Serial Number authorization only)

Windows 7

4GB RAM (667MHz)

1333MHz Xeon Core2Duo processor (or the fastest you can get)

Mid-range Nvidia video card (GForce 7300/7600)

Mac

10.4.1

Released 11-30-17

New Features

  • Search bar added to parameter selectors in the graph window.
  • Color mapped (aka “heat map statistic”) parameter labeling in layouts.

Bugs Fixed

  • Proliferation Platform Improvements
  • Stopped Compensation Wizard matrix re-calculations (ad infinitum).

More Info

For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes.

10.4

Released 09-15-17

New Features:

  • FlowJo Envoy integration now allows for seamless access to data in FlowJo Envoy workflows.
  • Hotly anticipated color mapping within the graph window, to illustrate a third parameter there is now available.
  • Signal spreading matrix (SSM) now available in the Compensation Editor.

Bugs Fixed:

  • Keywords now import into text-boxes in the Layout Editor.

Warnings:

  • When saving an ACS file from an FlowJo Envoy based workspace, the default location prompt is a temporary cache folder, which will be deleted when FlowJo is closed!
    • Workaround: Save FlowJo Envoy based workspace ACS files to a known, stable local directory.

More Info

For more about features and a list of known issues, see the exhaustive release notes.

10.3

Released 05-09-17

New Features:

  • We are happy to announce that the Proliferation Platform is here!
  • We have revised the Logicle Transform implementation. Existing workspaces with gates using this transform may need to be adjusted.

Warnings:

Because FlowJo® now uses Java 8, we no longer support Mac OS 10.6.8 (since it does not support Java 8).

Major Changes:

  • Proliferation Platform: FlowJo now supports Proliferation.
  • Revised Logicle Transform implementation to synchronize the user interface and the correct display ranges.
  • Java 8: FlowJo now uses Java 8, which brings a number of security and reliability improvements. This capability is now is distributed with the FlowJo install on both Windows and Mac.
  • Mac OS Sierra (10.12): This OS is now fully supported.

10.2

Released 09-02-16

Note: This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo.

Sierra, macOS 10.12, is now available as a free upgrade. This new version of the Mac OS supports Java 7 and higher. This update removes Java 6, which is required by FlowJo versions 10.2 and older. We are working on a new version of FlowJo™ that will use the current version of Java.

FlowJo can now use plugins that can perform a variety of functions, including the creation of populations, creation of derived parameters, and actions when opening or saving a workspace. 


The open plugin architecture of 10.2 allows access to cutting edge tools that can be integrated into your current analysis workflows, and we will be releasing developer documentation so any tool can be integrated into FlowJo. FlowJo 10.2 includes a set of tools for discovery including downsampling, tSNE and CellOntology. You'll find these as 'plugins' in the populations band -- each offers one-click access to powerful tools to reduce any number of parameters to two and to query a database to name unknown populations. 

New Features:

•Increased the number of parameters that FlowJo can analyze to 1000

•Added a field to allow specification of R's path in prefs (Diagnostic Panel)

•Better interface for Licensing Preferences Panel in the case where a computer has many hardware addresses.

•Updated EULA to raise the awareness of the fact that you may not install FlowJo on a VM.

•Unchecked 'Display other X/Y parameters than in parent plot' as default in Gates Preferences

•Removed the option to downsample when concatenating files. (Downsample before you concatenate if desired.)


Full Release Notes

9.9.6

Released 08-01-17

Release Notes:

  • Note: Workspace file format has changed – workspaces created in 9.9.6 cannot be opened in older versions of FlowJo.  (Save as XML to read in older versions)

Features:

  • Population comparison platforms allow selection of tSNE, LDA, and PCA parameters
  • New platforms:
    • Bivariate Difference Gating (BDG): Compare two parameter plots, such as tSNE maps, and gate on the differences.
    • List Value Gating: FCS files concatenated by FlowJo from multiple files can be sub-gated to select groups of files from similar samples.  For example: concatenate clinical specimens, and then gate on all data from only control subjects, or only female subjects, or any other criteria).
    • Mapped BDG: A combination of List Value Gating and BDG that allows you to compare and gate on differences between groups of subjects.

9.9.5

Released 12-11-17
  • Major Speed (3x) and Memory optimizations 
  • Polychromatic 'Y by Y' plot in the Layout Editor
  • tSNE can now be initialized from LDA or PCA parameters
  • Many other fixes and improvements
  • See full v9 release notes here!

9.9.4

Released 07-22-16

Minimum Requirements

Recommended Requirements

OSX 10.12.2 

Compatible with Mavericks

Native support for G3,4,5 and all Intel based systems.

2GB RAM

OSX 10.12.2+

*OSX 10.12.1 not currently supported*

8GB RAM or higher

New Dimension Reduction Platforms:

PCA (principal component analysis): http://docs.flowjo.com./v9/flowjo-v9-documentation-home/platforms/pca/

LDA (linear discriminant analysis -- optimal separation of multiple subsets) http://docs.flowjo.com/v9/flowjo-v9-documentation-home/platforms/lda/

Layout Editor accepts text pastes (and will create text object or table if tabular format)

Parameters selection for tSNE, LDA, PCA, and layout editor N x N plots made more powerful.

By default, most menus will no longer show uncompensated fluorescence parameters for compensated files. (Change this behavior by global preference, or at a single time by holding down the option key when clicking on the menu.)

Compensation Wizard displays the SSM with a heat map background to highlight parameter pairs with high spillover-spreading.

Sample Get Info has a new dialog window interface, with new capabilities (like showing a table of keywords in all samples in the group; showing only interesting keywords; showing only keywords with distinct values.)

FlowJo will notify the user if a Table or Layout Batch operation points to a group that has been deleted.

FlowJo now shows 9 decade log scale for Yeti files.

Fixed reading certain XML workspace files.

Fixed crash while editing compensation matrix (from v9.9.3)

8.8.7

Released 12-11-17

OSX 10.2 to 10.8 (will not work on OS X 10.9 and above).

512MB RAM, 2GB recommended.

USB2 port for dongle option.

Network adapter for Serial number option.

Network adapter and Internet access for site license option.Optimized for Intel and PPC Macs

Supports multiple processors, up to 8.

Improved Compensation Wizard

PolyChromatic Plot and PolyVariate plots

Molded and Spider Gates

Enhancements in Layout and Table editors

Custom formulas in the Table Editor

New Math Functions

Multiple Keyword Editing Options



7.6.5

Released 09-12-11

OSX 10.5 or higher

512MB RAM

USB port (dongle option)

Network adapter for an off-line V8 serial number

Network adapter and an Internet connection for Site License serial number.